Achieving Drought Tolerance in Rice by Targeted Genome Editing
Swagat Kumar Tripathy
Center for Biotechnology, Siksha ‘O’ Anusandhan Deemed to be University, Bhubaneswar, Odisha (751 003), India
Prasanta Kumar Majhi*
Dept. of Plant Breeding and Genetics, Regional Research and Technology Transfer Station, Odisha University of Agriculture and Technology, Keonjhar, Odisha (758 002), India
Ayaz Ahamad
Biosciences Group, Homi Bhabha National Institute, Bhabha Atomic Research Centre, Mumbai, Maharashtra (400 094), India
DOI: NIL
Keywords: CRISPR/Cas, Drought stress, Genome editing, Rice
Abstract
Rice is one of the major sources of food that feeds half of the world’s population, but the yield is significantly reduced due to several biotic and abiotic stresses. Among the abiotic stresses, drought is very severe which negatively affects plant growth, development and production, is a critical form of environmental stress. Drought stress affects diverse biochemical aspects, in addition to physiological and morphological traits, which are crucial for plant growth. Recent advancements in Genome Editing Technologies (GETs) involve the development of a clustered, regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated protein (Cas) system to achieve the desired goal. CRISPR-Cas-mediated genome editing has proven a dynamic tool for rapid and high-throughput reconfiguration of endogenous genes. Among these, the CRISPR/Cas9 system is the most efficient, easier, promising, reliable and widely adopted, for improving yield and stress resilience.
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Reference
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