Allele Mining: Modern Tools Of Biotechnology
Tarique Aslam*
Department of Vegetable Crops, BCKV, Mohanpur
O. J. Sujayasree
Department of PHT, UHS, Bagalkot
S. P. Das
Department of Vegetable Crops, BCKV, Mohanpur
A. K. Panda
Department of Fruit Science, TNAU, Tamil Nadu
DOI: NIL
Keywords: Allele mining, Tilling, Ecotilling, Sequencing
Abstract
Allele mining is the process of finding the superior allele from the natural population. In other words, introgression of novel or superior allele from wild relatives into cultivated one. Allele mining can be effectively used for discovery of superior alleles, through ‘mining’ the gene of interest from diverse genetic resources. It can also provide insight into molecular basis of novel trait variations and identify the nucleotide sequence changes associated with superior alleles. In addition, the rate of evolution of alleles; allelic similarity/dissimilarity at a candidate gene and allelic synteny with other members of the family can also be studied. Allele mining may also pave way for molecular discrimination among related species, development of allele-specific molecular markers, facilitating introgression of novel alleles through MAS or deployment through genetic engineering (GE).
Downloads
not found
Reference
Amaranatha, R. M., B. G. Vilas and R. T. Rajeswar. 2014. Allele Mining In Crop Improvement. Int J. Dev Res., 4(2): 300–305.
Till, B. J., S. H. Reynolds, E. A. Grene, C. A. Codomo, L. C. Enns, J. E. Johnson, C. Burtner, A. R. Odden, K. Young, N. E. Taylor, J. G. Henikoff, L. Comai and S. Henikoff. 2003. Large scale discovery of induced point mutations with high-throughput TILLING. Genome Res., 13: 524–530.