cDNA Library: A Tool for Molecular Plant Breeding
Niharika Yaduvanshi*
Dept. of Genetics and Plant Breeding, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, Faizabad, Uttar Pradesh (224 229), India
Ashim Debnath
Dept. of Genetics and Plant Breeding, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, Faizabad, Uttar Pradesh (224 229), India
Shiva Nath
Dept. of Genetics and Plant Breeding, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, Faizabad, Uttar Pradesh (224 229), India
Om Prakash Verma
Dept. of Genetics and Plant Breeding, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, Faizabad, Uttar Pradesh (224 229), India
Juhi Pandey
Dept. of Genetics and Plant Breeding, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, Faizabad, Uttar Pradesh (224 229), India
DOI: NIL
Keywords: cDNA, Construction, Molecular biology, Transcriptome
Abstract
By virtue of the powerful technology developed, it is possible to isolate after reverse transcription, any transcribed gene in the form of cDNA. cDNA library is a collection of cloned DNA fragments inserted into a collection of host cells, which together constitute some portion of the transcriptome of the organism. In recent years with development of molecular biology technology, the methods of construction of cDNA libraries have improved and enhanced greatly. In this article cDNA library construction was summarized and the applications cDNA library was described.
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Reference
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