CRISPR-Cas-based Detection of Plant Pathogens
Lham Dorjee*
Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi (110 012), India
Meniari Taku
Division of Genetics and Plant Breeding, ICAR-Indian Agricultural Research Institute, New Delhi (110 012), India
DOI: NIL
Keywords: CRISPR-Cas, Detection, Disease, Plant Pathogens
Abstract
Climate change is causing unforeseen alterations in pathogenic agents, contributing to the emergence of novel pathogen variants that can devastate crops. To overcome this, timely phytopathogen detection is crucial. CRISPR-Cas technology has recently emerged as a versatile and adaptable tool, well-suited for detecting a broad range of plant pathogens. This helps in developing disease management strategies and treatment plans. Among the different Cas variants, Cas12a and Cas13a are widely used for phytopathogen detection. Usually, the CRISPR-Cas system is followed after recombinase polymerase amplification or loop-mediated isothermal amplification, and the specific binding of the Cas protein to nucleic acid (Target) is detected as a measurable fluorescent or electrochemical signal. The advantages of CRISPR-Cas technology include high specificity, sensitivity, rapid results, etc. As CRISPR-Cas technology advances and becomes more accessible, integrating it into plant pathology holds great promise for improving food security and sustainability.
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Reference
Aman, R., Mahas, A., Marsic, T., Hassan, N., Mahfouz, M.M., 2020. Efficient, rapid, and sensitive detection of plant RNA viruses with one-pot RT-RPA-CRISPR/Cas12a assay. Frontiers in Microbiology 11, 610872. DOI: https://doi.org/10.3389/fmicb.2020.610872.
Jiao, J., Kong, K., Han, J., Song, S., Bai, T., Song, C., Wang, M., Yan, Z., Zhang, H., Zhang, R., Feng, J., Zheng, X., 2021. Field detection of multiple RNA viruses/viroids in apple using a CRISPR/Cas12a-based visual assay. Plant Biotechnology Journal 19(2), 394-405. DOI: https://doi.org/10.1111/pbi.13474.
Kang, H., Peng, Y., Hua, K., Deng, Y., Bellizzi, M., Gupta, D.R., Mahmud, N.U., Urashima, A.S., Paul, S.K., Peterson, G., Zhou, Y., Zhou, X., Islam, M.T., Wang, G.L., 2021. Rapid detection of wheat blast pathogen Magnaporthe oryzae Triticum pathotype using genome-specific primers and Cas12a-mediated technology. Engineering 7(9), 1326-1335. DOI: https://doi.org/10.1016/j.eng.2020.07.016.
Koonin, E.V., Makarova, K.S., 2019. Origins and evolution of CRISPR-Cas systems. Philosophical Transactions of the Royal Society B 374(1772), 20180087. DOI: https://doi.org/10.1098/rstb.2018.0087.
Wang, X., Shang, X., Huang, X., 2020. Next-generation pathogen diagnosis with CRISPR/Cas-based detection methods. Emerging Microbes & Infections 9(1), 1682-1691. DOI: https://doi.org/10.1080/22221751.2020.1793689.
Zhang, Y.M., Zhang, Y., Xie, K., 2020. Evaluation of CRISPR/Cas12a-based DNA detection for fast pathogen diagnosis and GMO test in rice. Molecular Breeding 40, 11. DOI: https://doi.org/10.1007/s11032-019-1092-2.
Zhang, Y.M., Yang, Y., Xie, K., 2021. CRISPR-Cas12a-based DNA detection for fast pathogen diagnosis and GMO test in plants. In: CRISPR-Cas Methods. (Eds.) Islam, M.T. and Molla, K.A. Springer Protocols Handbooks. Humana, New York, NY. pp. 221-233. DOI: https://doi.org/10.1007/978-1-0716-1657-4_15.