
CRISPR/Cas: A New Horizon in Plant Breeding
Avadhoot B. Dharmadhikari*
Dept. of Genetics and Plant Breeding, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur, Rajasthan (313 001), India
Aparna S.
Dept. of Genetics and Plant Breeding, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur, Rajasthan (313 001), India
DOI: NIL
Keywords: Breeding, CRISPR, Genetic modification, Genome editing
Abstract
Over the past few decades, the human population has increased significantly, raising serious concerns about the challenge of feeding a constantly expanding population. In such circumstances, increasing agricultural output is essential for providing humankind with both food and nourishment. Furthermore, because to their high time and labour requirements, standard breeding techniques are insufficient to supply this need. CRISPR-Cas enters the field of molecular approaches as a result to offer an alternative to the traditional techniques in order to overcome these conditions. Base editing, knock out or knock in of desirable genes, fine tuning of several genes, promotion of antiviral defence, and alteration of various demanding biochemical pathways through this specific gene editing system has proven its ability to spread new wings for accelerating crop advancement in last few years. Since the last few years, CRISPR has been significant in the advancement of studies involving genome editing.
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Reference
Doudna, J.A., Charpentier, E., 2014. The new frontier of genome engineering with CRISPR-Cas9. Science 346(6213), 258096. DOI: https://doi.org/10.1126/science.1258096.
Khatodia, S., Bhatotia, K., Passricha, N., Khurana, S.M.P., Tuteja, N., 2016. The CRISPR/Cas genome-editing tool: application in improvement of crops. Frontiers in Plant Science 7, 506. DOI: https://doi.org/10.3389/fpls.2016.00506.
Narayanan, N., Beyene, G., Chauhan, R.D., Gaitán-Solís, E., Gehan, J., Butts, P., Siritunga, D., Okwuonu, I., Woll, A., Jiménez-Aguilar, D.M., Boy, E., Grusak, M.A., Anderson, P., Taylor, N.J., 2019. Biofortification of field-grown cassava by engineering expression of an iron transporter and ferritin. Nature Biotechnology 37(2), 144-151. DOI: https://doi.org/10.1038/s41587-018-0002-1.
Zhang, A., Liu, Y., Wang, F., Li, T., Chen, Z., Kong, D., Bi, J., Zhang, F., Luo, X., Wang, J., Tang, J., Yu, X., Liu, G., Luo, L., 2019. Enhanced rice salinity tolerance via CRISPR/Cas9-targeted mutagenesis of the OsRR22 gene. Molecular Breeding 39, 1-10. DOI: https://doi.org/10.1007/s11032-019-0967-1.