Development of Low Cost Artificial Diet for Mass Production of Entomopathogenic Nematode, Heterorhabditis indica a Strain ICRI EPN-18
P. Thiyagarajan*
Indian Cardamom Research Institute, Spices Board India, Myladumpara, Idukki, Kerala (685 553), India
M. Varna
Indian Cardamom Research Institute, Spices Board India, Myladumpara, Idukki, Kerala (685 553), India
M.A. Ansar Ali
Indian Cardamom Research Institute, Spices Board India, Myladumpara, Idukki, Kerala (685 553), India
A.B. Rema Shree
Indian Cardamom Research Institute, Spices Board India, Myladumpara, Idukki, Kerala (685 553), India
DOI: https://doi.org/10.54083/PHA/1.2.2023/34-36
Keywords: Artificial diet, Elettaria cardamomum, Entomopathogenic nematode, Small cardamom
Abstract
Eight different diets were compared for maximum production of entomopathogenic nematode infected Galleria cadavers with cheaper/ low cost in the laboratory. Among eight artificial diet composition tried, low grade milk powder 100 g, low grade glycerin 100 ml, honey 100 ml, corn flour 200 g, wheat bran 100 g, wheat flour 100 g and yeast 50 g produced almost maximum Galleria larvae with EPN infected cadavers and EPN infective juveniles (IJs) with cheaper cost while, the diet containing, ragi powder 100 g instead of milk powder, honey 100 ml, high grade glycerin 100 ml, corn flour 200 g, wheat bran 100 g, wheat flour 100 g and yeast 50 g produced least number of EPN infected Galleria cadavers and EPN IJs. Among the diet compositions, sugar solution 100 ml instead of honey along with other ingredients used, did not emerge or produce larvae from the Galleria eggs.
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Reference
ICRI, 2008. Management of cardamom root grub, Basilepta fulvicorne (Jacoby) with entomopathogenic nematodes. Final report submitted to Department of Biotechnology, Ministry of Science & Technology, New Delhi, India. p. 215.
Desai, A., Siddhapara, M.R., 2020. Galleria mellonella L.: An important factitious host. In: ResearchGate. pp. 1-79. DOI: 10.13140/RG.2.2.21027.60963.
Liu, C.M., Shen, W.Y., Chen, K.Y., 1998. Comparison of growth and development of the greater wax moth Galleria mellonella reared on natural diets and an artificial diet. Wuyi Science Journal 14, 136-138.
Metwally, H.M.S., Hafez, G.A., Hussein, M.A., Hussein, M.A., Salem, H.A., Saleh, M.M.E., 2012. Low cost artificial diet for rearing the Greater Wax Moth, Galleria mellonella L. (Lepidoptera: Pyralidae) as a host for entomopathogenic nematodes. Egyptian Journal of Biological Pest Control 22(1), 15-17.
Varadarasan, S., Nagarajan, K., 2014. Studies on suitable formulation of entomopathogenic nematode for the management of cardamom root grub, Basilepta fulvicorne (Jacoby). Journal of Plantation Crops 42(2), 262-264.
Varadarasan, S., Hafitha, N.M., Sithara, L., Balamurugan, R., Chandrasekhar, S.S., Ansar Ali, M.A., Thomas, J., 2011. Entomopathogenic nematodes-science, technology and field outreach for biocontrol of cardamom root grub. Journal of Plantation Crops 39(1), 86-91.
Varadarasan, S., Sooravan, T., Chandrasekar, S.S., Ansar Ali, M.A., Thomas, J., 2006. Survey for Entomopathogenic Nematode in cardamom growing areas of Kerala and Tamil Nadu. Journal of Plantation Crops 34(3), 398-400.
Varna, M., 2017. Development of low cost artificial diet for rearing the greater wax moth, Galleria mellonella L. (Lepidoptera: Pyralidae) as a host for entomopathogenic nematode, the strain ICRI EPN-18. M.Sc. Dissertation, Mahatma Gandhi University, Kottayam, Kerala, India. p. 30.
White, G.F., 1927. A method for obtaining infective nematode larvae from cultures. Science 66(1709), 302-303.