Genome Editing as a Tool for Developing Disease Resistance in Rice
Sakthi A.R.*
Dept. of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
C. Selvi
Dept. of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
R. Poorniammal
Dept. of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
DOI: NIL
Keywords: CRISPR/Cas9, Disease resistance, Pathogen, Yield
Abstract
The clustered regularly interspaced short palindromic repeat (CRISPR)/ CRISPR-associated protein 9 nuclease (Cas9) system has recently emerged as an efficient and versatile tool for genome editing in various organisms. The Cas9 endonuclease from Streptococcus pyogenes, along with an artificial guide RNA (gRNA), is able to target the DNA sequence of 5′-N20-NGG-3′ (N indicates any base), in which N20 is the same as 20 bases of the gRNA and NGG is the protospacer-adjacent motif (PAM). CRISPR/Cas9 based genome editing was also used to develop indica rice lines with increased resistance to BLB by targeting the EBE sequence in promoter region. The blast resistant mutants were obtained by targeting ERF922 gene. The base editing approach was used to develop mutants which show resistance to rice tungro virus.
Downloads
not found
Reference
Kumam, Y., Rajadurai, G., Kumar, K.K., Varanavasiappan, S., Raveendran, M., Manonmani, S., Gopalakrishnan, C., Arul, L., Kokiladevi, E., Sudhakar, D., 2021. Adenine Base Editor Creates Novel Substitution Mutations in eIF4G Gene of Rice. Madras Agriculture Journal 107, 10-12. DOI: https://doi.org/10.29321/MAJ.10.000548.
Lee, J.H., Muhsin, M., Atienza, G.A., Kwak, D.Y., Kim, S.M., De Leon, T.B., Angeles, E.R., Coloquio, E., Kondoh, H., Satoh, K., Cabunagan, R.C., Cabauatan, P.Q., Kikuchi, S., Leung, H., Choi, I.R., 2010. Single nucleotide polymorphisms in a gene for translation initiation factor (eIF4G) of rice (Oryza sativa) associated with resistance to rice tungro spherical virus. Molecular Plant-Microbe Interaction 23(1), 29-38.
Ma, J., Chen, J., Wang, M., Ren, Y.L., Wang, S., Lei, C.L., Cheng, Z.J., Sodmergen, 2018. Disruption of OsSEC3A increases the content of salicylic acid and induces plant defense responses in rice. Journal Experimental Botany 69(5), 1051-1064.
Xu, Z.Y., Xu, X.M., Gong, Q., Li, Z.Y., Li, Y., Wang, S., Yang, Y.Y., Ma, W.X., Liu, L.Y., Zhu, B., Zou, L.F., Chen, G.Y., 2019. Engineering broad-spectrum bacterial blight resistance by simultaneously disrupting variable TALE-binding elements of multiple susceptibility genes in rice. Molecular Plant 12(11), 1434-1446.