Influence of Chemical-Induced Liberation of Pebrine Spores in Tasar Silkworm Mother Moth Examination
K. Nandhini*
Dept. of Sericulture, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh (515 003), India
Y. Praveen Reddy
Dept. of Sericulture, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh (515 003), India
U. Anil Kumar
Dept. of Sericulture, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh (515 003), India
M. Parasuramudu
Dept. of Sericulture, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh (515 003), India
DOI: https://doi.org/10.54083/PHA/1.3.2023/139-144
Keywords: Antheraea mylitta D., Pebrine, Mother moth examination, Sarcosyl
Abstract
Tasar silkworm suffers from various diseases especially Pebrine, Virosis, Bacteriosis and muscardine. Approximately 30-35% of crop losses can be attributed to Pebrine disease, with sporadic instances leading to complete crop failure. The primarily infection of Pebrine is by vertical transmission with the pathogen transferring the infection directly from parent to offspring. To ensure the perpetuation of Pebrine-free generations of silkworms, disease-free layings are produced through the examination of the mother moth. It is necessary to improve the visibility of spores by complete liberation, staining, cleaning of spores and dissolution/removal of fat bodies, debris of body tissues, etc. Certain chemicals are selected and tested the results revealed that, Sarcosyl, Formalin and Ethanol treated samples field was very clear and no debris was observed. Sarcosyl and Ethanol treated samples have shown 212,000 and 54,000 more number of pebrine spores cm-3 liberated when compared with K2CO3 (control) at 0.5% chemical concentration. Sarcosyl treated samples has shown 122,000 more number of pebrine spores cm-3 liberated when compared with K2CO3 (control) at 1% chemical concentration. Citric acid (300,000), Ethanol (100,000) and Sarcosyl (500,000) obtained more number of liberated pebrine spores cm-3 when compared with K2CO3 (control) at 2% chemical concentration. Sarcosyl (160,000), Ethanol (70,000) and Formalin (100,000) chemical treated samples obtained more number of liberated pebrine spores cm-3 when compared with K2CO3 (control) at 5% concentration. Sarcosyl, Formalin, Ethanol and Citric acid chemicals have shown better performance when compared with other tested chemicals and K2CO3 (control). Sarcosyl stands first position followed by Ethanol, Formalin and Citric acid chemicals in more number of liberation of pebrine spores cm-3.
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