Isolation and Identification of Soil Borne Fusarium oxysporum f. sp. lentis
Ashwini E.*
Dept. of Plant Pathology, Jawaharlal Nehru Krishi Vishwavidyalaya (JNKVV), Jabalpur, Madhya Pradesh (482 004), India
Dinkwar G.T.
Dept. of Plant Pathology, Jawaharlal Nehru Krishi Vishwavidyalaya (JNKVV), Jabalpur, Madhya Pradesh (482 004), India
Nandeesha S.V.
Dept. of Plant Pathology, Jawaharlal Nehru Krishi Vishwavidyalaya (JNKVV), Jabalpur, Madhya Pradesh (482 004), India
DOI: NIL
Keywords: F. oxysporum f. sp lentis, Fingerprinting, Lentil, PCR
Abstract
In India, lentil ranks second most important pulse crop next to chickpea both in area and production. All about 80,000 diseases have been recorded throughout the world, of them majority are associated with soil-borne diseases. Among Soil-borne diseases lentil wilt caused by Fusarium oxysporum f. sp. lentis is most destructive pathogen causes yield loss up to 50% in farmer’s fields The pathogen was isolated from soil as well as from infected plant sample. The most commonly used media for isolation of F. oxysporum f. sp lentis is Potato dextrose agar medium (PDA), it is nutrient rich media for growing wide range of fungi and the method used for isolation from soil is dilution for pour plating at 10-4 conc. The pathogen was detected after obtaining pure culture, primarily through cultural characters, microscopic observation of micro and macro conidia and by using molecular methods like DNA finger printing and PCR based methods.
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Reference
Ainsworth, G.C., 1971. Ainsworth and Bisby’s Dictionary of the Fungi. 6th Ed. Commonwealth Mycological Institute, Kew Surrey, England, p. 663.
Goodwin, P.H., English, J.T., Neher, D.A., Diniway, J.M., 1990. Detection of Phytophthora parasitica from soil and host tissue with a species-specific DNA probe. Phyto Pathol 80, 277-281.
Kumar, V., Ninama, A., Khande, D., Amrute, S., Pandey, S.K., Pandey, S., 2019. Current scenario of lentil production in Madhya Pradesh. I. J. Agri. Sci 11, 9061-9063.
Rahman, T., Ahmed, A.U., Islam, M.R., Hosen, M.I., 2010. Physiological study and both in vitro and in vivo antifungal activities against Stemphylium botryosum causing Stemphylium blight disease in lentil (Lens culinaris). J. Plant Pathol 9, 179-187.
Tsugunori, N., Hiroto, O., Harumi, M., Keiko, W., Nobuyuki, A., Tetsu, H., 2000. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28(12), 63.