Loop Mediated Isothermal Amplification (LAMP) - A Robust Molecular Tool for Rapid Disease Diagnosis
Rajesh, S.*
Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
Varanavasiappan, S.
Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
Dhivya, K.
Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
DOI: NIL
Keywords: Disease diagnostics, Gene amplification, LAMP, PCR
Abstract
Genetic testing is widely used method for disease diagnosis in human, animal and plant systems. Currently, PCR is a method of choice for gene amplification but requires a thermal cycler for the reaction and electrophoresis by fluorescent intercalator to detect the amplification. However, this method remains elusive only to affordable laboratories across world. Loop-mediated isothermal amplification (LAMP) is an outstanding gene amplification procedure that combines rapidity, simplicity, and high specificity. Samples are amplified at a fixed temperature through a repetition of two types of elongation reactions occurring at the loop regions. LAMP technique has wide applications, including in point-of-care testing, genetic testing in resource constrained places, and rapid testing of food products and environmental samples.
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Reference
Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., and Hase, T., 2000.Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28, E63.
Tomita, N., Mori, Y., Kanda, H., and Notomi, T.,2008.Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products.Nat. Protoc. 3, 877–882.
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