Metabarcoding: A Molecular Phylogenetic Tool for Large scale, Rapid Assessment of Species Diversity
Rajesh, S.*
Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
Sudha, M.
Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
Varanavasiappan, M.
Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
Jayabalakrishnan, R. M.
Directorate of Natural Research Management, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
DOI: NIL
Keywords: Biodiversity, Ecology, Metabarcoding, Phylogenetics
Abstract
Metabarcoding is a DNA barcoding process that allows for the simultaneous identification of several taxa within the same sample. This technique can be synonymously applied to environmental DNA (eDNA) barcoding. Primary difference of this technique and the barcoding is that metabarcoding does not focus on one specific organism whereas it aims to determine species composition within a sample. Unlike DNA barcoding, metabarcoding makes use of Next Generation Sequencing approaches for rapid assessment of species diversity.
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Reference
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