Nanopore Sequencing for Diagnosis and Resistance Profiling of Pathogens
Zahoor Mushtaq*
Aquatic Environment and Health Management Division, ICAR-CIFE, Mumbai, Maharashtra (400 061), India
Kurcheti Pani Prasad
Aquatic Environment and Health Management Division, ICAR-CIFE, Mumbai, Maharashtra (400 061), India
Ubaid Qayoom
Fish Genetics and Biotechnology Division, ICAR-CIFE, Mumbai, Maharashtra (400 061), India
DOI: NIL
Keywords: Aquaculture, MinIONTM, Portable, Third-generation
Abstract
Effective treatment and preventive measures of infectious diseases demand rapid and accurate identification of causative agents. Oxford nanopore MinION™ is a commercially available portable, convenient, relatively fast, and cost-effective DNA sequencer providing sequencing data in real-time. MinION™ utilizes the base-specific fluctuations due to blockage of a nanopore and ultimately transforming it into DNA sequence information. Nanopore MinION™ has been used worldwide to deliver new insights and real-time results for a broad range of applications, which include epidemiological surveillance programs, field diagnostics, microbiome identification. Long read Oxford Nanopore MinION™ whole-genome sequencing has been accurately used for sequence typing and determining antibiotic resistance profiles of various pathogens. Oxford nanopore MinION™ sequencing offers a vast potential for cost-effective, rapid whole-genome sequencing for pathogen diagnosis and resistance identification in real-time.
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Reference
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