Phospholipid Fatty Acid Profiling for Soil Microbial Community Analysis in Soil Conservation Farming, Missouri
Sougata Bardhan*
The Center for Agroforestry, School of Natural Resources, 203 Anheuser-Busch Natural Resources Building, University of Missouri, MO 65211, Columbia
School of Natural Resources, 302 Anheuser-Busch Natural Resources Building, University of Missouri, MO 65211, Columbia
Ranjith P. Udawatta
The Center for Agroforestry, School of Natural Resources, 203 Anheuser-Busch Natural Resources Building, University of Missouri, MO 65211, Columbia
School of Natural Resources, 302 Anheuser-Busch Natural Resources Building, University of Missouri, MO 65211, Columbia
Shibu Jose
The Center for Agroforestry, School of Natural Resources, 203 Anheuser-Busch Natural Resources Building, University of Missouri, MO 65211, Columbia
School of Natural Resources, 302 Anheuser-Busch Natural Resources Building, University of Missouri, MO 65211, Columbia
Clark J. Gantzer
School of Natural Resources, 302 Anheuser-Busch Natural Resources Building, University of Missouri, MO 65211, Columbia
Christopher Bobryk
Missouri Department of Natural Resources, 1101 Riverside Drive, Jefferson City, MO 65101, Missouri
DOI: NIL
Keywords: Bacteria, Cover crop, Crop rotation, Diversity, PLFA
Abstract
Cover crops and crop rotations are utilized for improving degraded soils by creating a continuous ground cover and adding organic matter. They improve soil microbial biodiversity which is also an indicator of soil health. The objective of this study was to evaluate the impact of cover crops and crop rotation on soil microbial properties using phospholipid fatty acid (PLFA) profiles. Corn (Zea mays L.)/soybean (Glycine max. L), (CS) and corn/soybean/wheat (Triticum aestivum L.) (CSW) rotation with various cover crop mixtures was tested. The control treatment had no cover crops. The PLFA profile results indicated that total bacteria (3331-1487 ng g-1 soil), fungi (980-355 ng g-1 soil), protozoa (111-25 ng g-1 soil), actinomycetes (613-263 ng g-1 soil) were significantly higher in CSW plots compared to CS rotation plots and control, indicating the impact on soil microbial population. The short period of cover crop incorporation did not influence soil microbial population significantly.
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