Plant Gene Editing Approaches for Enhancing Pest and Disease Resistance
B.G. Sangeetha*
Division of Crop Protection, ICAR-Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram, Kerala (605 017), India
T. Makeshkumar
Division of Crop Protection, ICAR-Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram, Kerala (605 017), India
E.R. Harish
Division of Crop Protection, ICAR-Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram, Kerala (605 017), India
DOI: NIL
Keywords: Pest, Gene editing, Transcriptomics, Disease
Abstract
The crop plants have been domesticated from 10000 years ago and conventional breeding methods were used for developing new crop varieties. With the development of new sequencing technologies about 300 of reference plant genome is available in various nucleotide database and diversity of crop wild relatives (CWRs) genome can be utilized for developing new cultivars. The CRISPR/Cas9 technology has been successfully applied across a range of crops, including rice, wheat, tomato, potato, tobacco and maize. The gene edited plants can be developed by inserting mutations at target sites in plant genome without inclusion of foreign gene. A list of genome editing techniques have been adapted for use in plant genomes, such as homologous recombination (HR), ZFNs, TALENs, PPRs, the CRISPR/Cas9 system, RNAi, as well as cisgenesis and intragenesis.
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Reference
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