Protein Linkers: Catalysts for Innovations in Recombinant Fusion Proteins across Industries
Berryish Metha C.*
Dept. of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
Samuel Peter
Dept. of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
Bishnupriya Gauda
Dept. of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
Dhanvarsha M.
Dept. of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
DOI: NIL
Keywords: Agricultural improvement, Pharmacokinetic profiles, Protein linkers, Recombinant fusion protein
Abstract
This article explores the pivotal role of protein linkers in constructing stable and bioactive recombinant fusion proteins, showcasing their diverse applications across industries. Three linker categories: flexible, rigid and in vivo cleavable; address specific construction needs. Flexible linkers, rich in small or polar amino acids, offer mobility for interacting domains. Rigid linkers, characterized by α-helices or proline-rich sequences, efficiently separate functional domains. Cleavable linkers enable in vivo domain release, enhancing chimeric protein bioactivity and targeted drug delivery. Linker selection criteria consider system-specific nuances and contribute to the strategic construction of functional chimeric proteins. Linkers enhance folding and stability and modulate expression levels, influencing bioactivity and pharmacokinetic profiles. Fusion proteins find diverse applications, from protein purification to cancer diagnostics and agricultural improvement. Effective linkers contribute to drug conjugate efficiency, exemplified by Fc-fusion proteins. Overall, the review highlights linkers' critical role in advancing recombinant fusion proteins across scientific and medical domains.
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Reference
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