Real-Time PCR: A Sensitive, Specific and Rapid Diagnostics Assay of Fish and Shellfish Pathogens
Pratapa M.G.
ICAR-Central Institute of Fisheries Education (CIFE), Mumbai, Maharashtra (400 061), India
David Waikhom*
ICAR-Central Institute of Fisheries Education (CIFE), Mumbai, Maharashtra (400 061), India
Laishram Soniya Devi
College of Fisheries, Central Agricultural University (Imphal), Lembucherra, Tripura (799 210), India
DOI: NIL
Keywords: Diagnostics, Pathogens, Real-time PCR, SYBR green
Abstract
The real-time PCR (polymerase chain reaction) assay is one of the most commonly used techniques in disease diagnostics. This advanced form of PCR has both pros and cons. Real-time PCR has advantages over conventional PCR in terms of specificity, rapidity, and sensitivity to identify a pathogen at the genomic level. It has many forms of chemistry, such as SYBR green-based, probe-based, etc., based on its working principle. Many researchers have reported that real-time PCR could accurately detect and identify various fish pathogens. However, it has some drawbacks, such as the high cost and required a skilled person to operate this assay.
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Reference
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