Recent Advances on CRISPR/Cas9 based Genome Editing in Silkworms Bombyx mori L. (Lepidoptera: Bombycidae)
Sangeeta Dash
Division of Entomology, ICAR-IARI, New Delhi, Delhi (110 012), India
DOI: NIL
Keywords: CRISPR/Cas9, Genetics, Silk, Silkworms
Abstract
Bombyx mori, the mulberry silkworm, is of the utmost significance in the discipline of sericulture due to extensive production of glossy silk fibres. Apart from playing a crucial role in the textile industry, it is also a model organism for various scientific studies and a bioreactor that facilitates the production of recombinant protein. Therefore, numerous efforts are being made to appropriately alter silkworm genetics. The infamous CRISPR/Cas9 genome editing technique is currently in use for manipulation of vital genes as BmBlos2, BmKu70, BmFibH, BmCactus, BmJhe, immediate early-1 (ie-0) and (ie-2) in B. mori. Therefore, this review briefly highlights the advancements made to manipulate the genome of silkworms in the current decade using CRISPR/Cas9 as a tool in hand. It is also understood as to how CRISPR-Cas9 systems heightened the basic research on B. mori and other organisms, demonstrating the enormous promise of insect biotechnology across a wide range of disciplines.
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Reference
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