Seroreactivity Assay of Crude and Fractionated Outer Membrane Protein of Aeromonas sobria Isolated from Goldfish (Carassius auratus Linnaeus, 1758)
Prasenjit Mali*
Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, West Bengal, INDIA;
Koel Bhattacharya Sanyal
National Surveillance Programme for Aquatic Animal Diseases, West Bengal University of Animal and Fishery Sciences, West Bengal, INDIA;
Debapriyo Mukherjee
National Surveillance Programme for Aquatic Animal Diseases, West Bengal University of Animal and Fishery Sciences, West Bengal, INDIA;
Avijit Biswas
Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, West Bengal, INDIA;
Biswadeep Dey
Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, West Bengal, INDIA;
T. J. Abraham
Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, West Bengal, INDIA;
Gadadhar Dash
Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, West Bengal, INDIA;
S.N. Joardar
Department of Veterinary Microbiology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, West Bengal, INDIA
DOI: NIL
Keywords: Aeromonas sobria, outer membrane protein, sero diagnosis, gold fish
Abstract
Aeromonas sobria has frequently been reported as a causative agent of motile Aeromonas septicemia (MAS) along with A. hydrophila in fish and other aquatic organisms. Till now there is lack of precise tool for early diagnosis of this disease. The aim of the present study was to fractionate and characterize the outer membrane protein (OMP) antigen of A. sobria by serological techniques so as to identify immunoreactive molecules that might be useful in preparing immunodiagnostic tools against A. sobria infection in goldfish. Eight fractions were isolated from the crude OMP antigen using Sephacryl S-200 and DEAE-cellulose chromatography. The highest seroreactivity was observed in the gel-permeated protein G1 which had an optical density (OD) of 0.72 nm, higher even than that of the crude OMP antigen, 0.63 nm. The serodiagnostic potential of G1 was assessed by using dip-stick ELISA. Therefore, fractionated antigen G1 (molecular wt 42-67 kDa) should be further studied in immunodiagnostic tool preparations for A. sobria infection.
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