Silver Nanoparticles for Improved Genetic Transformation Efficiency
Swapna Alex*
Dept. of Plant Biotechnology, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala Agricultural University, Kerala (695 522), India
Amala Benny
Dept. of Plant Biotechnology, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala Agricultural University, Kerala (695 522), India
Nitasana Rajkumari
Dept. of Plant Biotechnology, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala Agricultural University, Kerala (695 522), India
K.B. Soni
Dept. of Plant Biotechnology, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala Agricultural University, Kerala (695 522), India
K.N. Anith
Dept. of Agricultural Microbiology, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala Agricultural University, Kerala (695 522), India
DOI: NIL
Keywords: Agrobacterium, Biolistics, Genetic transformation, Silver nanoparticles
Abstract
Important applications of silver nanoparticles are reported in healthcare, agriculture, food packaging, textiles, etc. The unique physico-chemical and biological properties of silver nanoparticles including antimicrobial property which is attributed to their property to alter the membrane permeability indicate their potential for improvement of plant genetic transformation techniques also. Silver nanoparticles of 100 nm can be effectively used for biolistic and Agrobacterium mediated genetic transformation without cytotoxic effect and are more efficient than conventional techniques. A ten-fold increase in the transformation efficiency of Agrobacterium and 37.5 fold decreases in the cost of consumables could be observed with optimised concentrations of 100 nm silver nanoparticles compared to conventional calcium chloride freeze thaw technique of transformation of Agrobacterium tumefaciens and gold microcarrier mediated biolistic transformation of Nicotiana tabacum respectively.
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Reference
Benny, A., Alex, S., Soni, K.B., Anith, K.N., Kiran, A.G., Viji, M.M., 2022. Improved transformation of Agrobacterium assisted by silver nanoparticle. BioTechnologia 103(3), 311-317.
Rajkumari, N., Alex, S., Soni, K.B., Anith, K.N., Viji, M.M., Kiran, A.G., 2021. Silver nanoparticles for biolistic transformation in Nicotiana tabacum L. 3 Biotech 11(12), 497.