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2022-11-13

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Santhoshinii, E., Jahagirdar, S., Priya, S.D., Siva, M., Pothiraj, G., 2022. Spray Induced Gene Silencing (SIGS): A new domain in plant disease management. Biotica Research Today 4(11), 775-777.

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HOME / ARCHIVES / Vol. 4 No. 11 : November (2022) / Popular Article

Spray Induced Gene Silencing (SIGS): A New Domain in Plant Disease Management

Santhoshinii E.*

Dept. of Plant Pathology, University of Agricultural Sciences, Dharwad, Karnataka (580 005), India

Shamarao Jahagirdar

Dept. of Plant Pathology, University of Agricultural Sciences, Dharwad, Karnataka (580 005), India

Shanmuga Priya D.

Dept. of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India

Siva M.

Dept. of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India

Pothiraj G.

Nammazhvar College of Agriculture and Technology, Kamuthy, Ramanathapuram, Tamil Nadu (623 708), India

DOI: NIL

Keywords: Double stranded RNA (dsRNA), Nanoparticles, RNA interference (RNAi), Spray Induced Gene Silencing (SIGS)

Abstract


Gene silencing is a negative feedback mechanism which is used in plants against diseases and crop improvement. Sustainable management of crop diseases demand novel, eco-friendly, cost effective and transgenic free strategies. An alternative approach is need of hour to avoid chemical fungicides, to overcome conventional breeding and transgenic methods. The RNA interference (RNAi) is a natural defence mechanism against foreign nucleic acids, transposons and viruses which is found in all eukaryotic organisms. The Host Induced Gene Silencing (HIGS) is not popular mainly because of the public concerns for the consumption of genetically modified crops and instability of RNA silenced genetically engineered crops. A recent and novel discovery, Spray Induced Gene Silencing (SIGS) is based on RNAi mechanism which uses the topical application of pathogen specific double stranded RNA (dsRNA) on plant surface that confers disease resistance in plants. This sprayable RNA with nanotechnology is an effective sustainable strategy for crop protection.

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Reference


Dubrovina, A.S., Kiselev, K.V., 2019. Exogenous RNAs for Gene Regulation and Plant Resistance. International Journal of Molecular Sciences 20 (9), 2282. DOI: http://dx.doi.org/10.3390/ijms20092282.

Liu, S., Geng, S., Li, A., Mao, Y., Mao, L., 2021. RNAi technology for plant protection and its application in wheat. aBIOTECH 2, 365-374. DOI: https://doi.org/10.1007/s42994-021-00036-3.

Mitter, N., Worrall, E.A.., Robinson, K.E., Li, P., Jain,R.G., Taochy, C., Fletcher, S.J., Carroll, B.J., Lu, G.Q., Xu, Z.P., 2017. Clay nanosheets for topical delivery of RNAi for sustained protection against plant viruses. Nature Plants 3(2), 1-10. DOI: http://dx.doi.org/10.1038/nplants.2016.207.

Qiao, L., Lan, C., Capriotti, L., Ah-Fong, A., Sanchez, J.N., Hamby, R., Heller, J., Zhao, H., Glass, N.L., Judeson, H.S., Mezzetti, B., Niu, D., Jin, H., 2021. Spray-induced gene silencing for disease control is dependent on the efficiency of pathogen RNA uptake. Plant Biotechnology Journal 19(9), 1756-1768. DOI: 10.1111/pbi.13589.

Uslu, V.V., Wassenegger, M., 2020, Critical view on RNA silencing- mediated virus resistance using exogenously applied RNA. Current Opinion in Virology 42, 18-24. DOI: https://doi.org/10.1016/j.coviro.2020.03.004.