Studies on Cultural and Morphological Variability in the Isolates of Fusarium solani Causing Dieback Disease in Tea Ecosystem of NE India
Pritam Das
School of Crop Protection, CPGS-AS, CAU (Imphal), Umiam, Meghalaya (793 103), India
Madhusmita Mahanta
School of Crop Protection, CPGS-AS, CAU (Imphal), Umiam, Meghalaya (793 103), India
Abhigyan Bhattacharyya
School of Crop Protection, CPGS-AS, CAU (Imphal), Umiam, Meghalaya (793 103), India
Pranab Dutta*
School of Crop Protection, CPGS-AS, CAU (Imphal), Umiam, Meghalaya (793 103), India
DOI: https://doi.org/10.54083/PHA/3.1.2025/01-07
Keywords: Cultural characteristics, Dieback disease, Fusarium solani, Morphological characteristics, Tea
Abstract
Total 10 Fusarium solani were isolated and coded as NMFst1, NMFst2, NMFst3, NMFst4, UMFst1, UMFst2, UMFst3, PAFst, SDFst and CDFst from the tea ecosystem of three North Eastern states of India viz., Meghalaya, Assam and Arunachal Pradesh. The isolates were characterized based on cultural characteristics on colony colour (as per Royal Horticultural Society colour chart), zonation (present or absent), topography (raise and umbonate) and sporulation (profuse or moderate) were studied. Morphological characteristics of the isolated Fusarium solani grown on PDA medium and microscopic observations were made under 40X. Size of the macro conidia and for the microconidia were found to be in the range of 5.79-23.50×1.29-2.33 μm and 3.12-8.58×1.22-1.88 μm, respectively. Macro conidia are found to be sickle shape (all the isolates) with blunt end (PAFst) and microconidia were round to oval (all isolates) in shape. The colour of mycelium was found to be hyaline. Presence of septation in macroconidia (5-3) and mycelium were also recorded. By comparing the cultural and morphological characteristics with the key guidelines of C. Booth (1971), species level identification was done. Screening for the first growing isolate of Fusarium solani was done by taking the radial growth of the isolates at different days after incubation (up to 168 hours). Among the 10 isolates, 6 isolates were found to be fast growing in nature.
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