TILLING: A novel reverse genetics technique for screening of gene mutants in plants
Bichhinna Maitri Rout
Division of Vegetable Science, Ph.D. Scholar, ICAR-IARI, New Delhi-110012, India
DOI: NIL
Keywords: TILLING, reverse genetics, endonuclease, mutation
Abstract
TILLING is a newly developed general reverse genetics strategy which helps to locate an allelic series of induced point mutations in genes of interest and allows the rapid and inexpensive detection of induced point mutations in populations of physically or chemically mutagenized individuals. This approach works with a mismatch-specific endonuclease to detect induced or natural DNA polymorphisms in desired genes.
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Reference
Chen, L., Hao, L., Parry, M. A., Phillips, A. L., & Hu, Y. G. (2014). Progress in TILLING as a tool for functional genomics and improvement of crops. Journal of integrative plant biology, 56(5), 425-443.
Esfeld, K., Uauy, C., & Tadele, Z. (2013). Application of TILLING for orphan crop improvement. In Biotechnology of Neglected and Underutilized Crops (pp. 83-113). Springer, Dordrecht.
Khan, A., Abidi, I., Bhat, M. A., Dar, Z. A., Ali, G., Shikari, A. B., & Khan, M. A. (2018). Tilling and Eco-Tilling–A Reverse Genetic Approach for Crop Improvement. Int. J. Curr. Microbiol. App. Sci, 7(6), 15-21.