Loop Mediated Isothermal Amplification (LAMP) - A Robust Molecular Tool for Rapid Disease Diagnosis

Abstract

Genetic testing is widely used method for disease diagnosis in human, animal and plant systems. Currently, PCR is a method of choice for gene amplification but requires a thermal cycler for the reaction and electrophoresis by fluorescent intercalator to detect the amplification. However, this method remains elusive only to affordable laboratories across world. Loop-mediated isothermal amplification (LAMP) is an outstanding gene amplification procedure that combines rapidity, simplicity, and high specificity. Samples are amplified at a fixed temperature through a repetition of two types of elongation reactions occurring at the loop regions. LAMP technique has wide applications, including in point-of-care testing, genetic testing in resource constrained places, and rapid testing of food products and environmental samples.