cDNA Library: A Tool for Molecular Plant Breeding
Keywords:
cDNA, Construction, Molecular biology, TranscriptomeAbstract
By virtue of the powerful technology developed, it is possible to isolate after reverse transcription, any transcribed gene in the form of cDNA. cDNA library is a collection of cloned DNA fragments inserted into a collection of host cells, which together constitute some portion of the transcriptome of the organism. In recent years with development of molecular biology technology, the methods of construction of cDNA libraries have improved and enhanced greatly. In this article cDNA library construction was summarized and the applications cDNA library was described.