Rice Genome Editing through CRISPR/Cas9: Where are We?

Abstract

By application of various conventional, mutational and molecular breeding approaches considerable improvement and high productivity of Rice (Oryza sativa L.) have been gained. However, these methods are not enough to enhance the yield as well as combating the resurgence of various biotic and extending abiotic stress conditions. So, now-a-days to boost up these factors, advanced genome editing tool like CRISPR/cas9 is on high demand. This system uses a complex of RNA-Protein (sgRNA-Cas9) to proceed with a double-strand break in the target DNA, a repair mechanism (NHEJ or HDR) and direct knocking out of the target gene. Even it can replace a desirable host gene in that position to enhance the desirable trait.