Characterization of Traditional Rice Varieties for Leaf Blast Resistant Genes Pi5, Pi54, Pi9 and Pi2 using Gene Specific Markers

Authors

  • A. Shanmugam Dept. of Genetics and Plant Breeding, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
  • R. Suresh Dept. of Genetics and Plant Breeding, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
  • A. Ramanathan Agricultural Research Station, Kattuthottam, Thanjavur, Tamil Nadu (613 501), India
  • P. Anandhi Tamil Nadu Rice Research Institute, Aduthurai, Thanjavur, Tamil Nadu (612 101), India
  • R. Pushpa Tamil Nadu Rice Research Institute, Aduthurai, Thanjavur, Tamil Nadu (612 101), India
  • D. Sassikumar Sugarcane Research Station, Cuddalore, Tamil Nadu (607 001), India

DOI:

https://doi.org/10.54083/ResBio/5.4.2023/158-161

Keywords:

Gene linked markers, Functional marker, Blast, Traditional varieties

Abstract

Magnaporthe oryzae poses a serious risk to rice growing regions worldwide. To combat this, future breeding efforts that aim to develop resistant varieties will need to identify and screen blast-resistant cultivars from existing germplasms. Thus, present study aimed to identify four major blast resistant genes (Pi54, Pi5, Pi2 and Pi9) in 20 traditional rice varieties using functional and linked markers. Results of the present study identified that fifteen traditional rice landraces were found to possess at least one resistant gene and three traditional landraces (Aanaikomban, Chenellu and Jai Sri Ram) had two resistant genes. These identified traditional rice landraces could be used as promising donor against rice blast disease for future rice breeding programmes to develop superior cultivars.

Downloads

Download data is not yet available.

References

Dean, R.A., Talbot, N.J., Ebbole, D.J., Farman, M.L., Mitchell, T.K., Orbach, M.J., Thon, M., Kulkarni, R., Xu, J., Pan, H., Read, N.D., Lee, Y., Carbone, I., Brown, D., Oh, Y.Y., Donofrio, N., Jeong, J.S., Soanes, D.M., Djonovic, S., Kolomiets, E., Rehmeyer, C., Li, W., Harding, M., Kim, S., Lebrun, M., Bohnert, H., Coughlan, S., Butler, J., Calvo, S., Ma, L., Nicol, R., Purcell, S., Nusbaum, C., Galagan, J.E., Birren, B.W., 2005. The genome sequence of the rice blast fungus Magnaporthe grisea. Nature 434, 980-986. DOI: https://doi.org/10.1038/nature03449. DOI: https://doi.org/10.1038/nature03449

Doyle, J.J., Doyle, J.L., 1990. Isolation of plant DNA from fresh tissue. Focus 12(1), 13-15. DOI: https://doi.org/10.2307/2419362

Fjellstrom, R., Conaway-Bormans, C.A., McClung, A.M., Marchetti, M.A., Shank, A.R., Park, W.D., 2004. Development of DNA markers suitable for marker assisted selection of three Pi genes conferring resistance to multiple Pyricularia grisea pathotypes. Crop Science 44(5), 1790-1798. DOI: https://doi.org/10.2135/cropsci2004.1790. DOI: https://doi.org/10.2135/cropsci2004.1790

Han, S.S., 2001. Improvement of disease evaluation system and classification of rice blast isolates. In: Proceedings of the First Workshop on Rice Blast. National Institute of Crop Science, Milyang, Korea. pp. 1-19.

Hasan, M.M., Rafii, M.Y., Ismail, M.R., Mahmood, M., Rahim, H.A., Alam, M.A., Ashkani, S., Malek, M.A., Latif, M.A., 2015. Marker-assisted backcrossing: A useful method for rice improvement. Biotechnology and Biotechnological Equipment 29(2), 237-254. DOI: https://doi.org/10.1080/13102818.2014.995920. DOI: https://doi.org/10.1080/13102818.2014.995920

Ingole, K.D., Prashanthi, S.K., Krishnaraj, P.U., 2014. Mining for major blast resistance genes in rice landraces of Karnataka. Indian Journal of Genetics and Plant Breeding 74(3), 378-383. DOI: https://doi.org/10.5958/0975-6906.2014.00857.8. DOI: https://doi.org/10.5958/0975-6906.2014.00857.8

Jain, P., Dubey, H., Singh, P.K., Solanke, A.U., Singh, A.K., Sharma, T.R., 2019. Deciphering signalling network in broad spectrum Near Isogenic Lines of rice resistant to Magnaporthe oryzae. Scientific Reports 9, 16939. DOI: https://doi.org/10.1038/s41598-019-50990-8. DOI: https://doi.org/10.1038/s41598-019-50990-8

Jeevan, B., Hosahatti, R., Koti, P.S., Devappa, V.H., Ngangkham, U., Devanna, P., Yadav, M.K., Mishra, K.K., Aditya, J.P., Boraiah, P.K., Gaber, A., Hossain, A., 2023. Phenotypic and genotypic screening of fifty-two rice (Oryza sativa L.) genotypes for desirable cultivars against blast disease. PLoS ONE 18(3), e0280762. DOI: https://doi.org/10.1371/journal.pone.0280762. DOI: https://doi.org/10.1371/journal.pone.0280762

Jeon, J.S., Chen, D., Yi, G.H., Wang, G.L., Ronald, P.C., 2003. Genetic and physical mapping of Pi5(t), a locus associated with broad-spectrum resistance to rice blast. Molecular Genetics and Genomics 269, 280-289. DOI: https://doi.org/10.1007/s00438-003-0834-2. DOI: https://doi.org/10.1007/s00438-003-0834-2

Jiang, N., Li, Z., Wu, J., Wang, Y., Wu, L., Wang, S., Wang, D., Wen, T., Liang, Y., Sun, P., Liu, J., Dai, L., Wang, Z., Wang, C., Luo, M., Liu, X., Wang, G.L., 2012. Molecular mapping of the Pi2/9 allelic gene Pi2-2 conferring broad-spectrum resistance to Magnaporthe oryzae in the rice cultivar Jefferson. Rice 5, 29. DOI: https://doi.org/10.1186/1939-8433-5-29. DOI: https://doi.org/10.1186/1939-8433-5-29

Khamari, B., 2020. Grain discolouration: An emerging threat to rice crop. Research Biotica 2(2), 80-87. DOI: https://doi.org/10.54083/ResBio.2.2.2020.80-87. DOI: https://doi.org/10.54083/ResBio.2.2.2020.80-87

Khan, G.H., Shikari, A.B., Vaishnavi, R., Najeeb, S., Padder, B.A., Bhat, Z.A., Parray, G.A., Bhat, M.A., Kumar, R., Singh, N.K., 2018. Marker-assisted introgression of three dominant blast resistance genes into an aromatic rice cultivar Mushk Budji. Scientific Reports 8, 4091. DOI: https://doi.org/10.1038/s41598-018-22246-4. DOI: https://doi.org/10.1038/s41598-018-22246-4

Lee, S.K., Song, M.Y., Seo, Y.S., Kim, H.K., Ko, S., Cao, P.J., Suh, J.P., Yi, G., Roh, J.H., Lee, S., An, G., Hahn, T.R., Wang, G.L., Ronald, P., Jeon, J.S., 2009. Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes. Genetics 181(4), 1627-1638. DOI: https://doi.org/10.1534/genetics.108.099226. DOI: https://doi.org/10.1534/genetics.108.099226

Mahender, A., Swain, D.M., Gitishree, D., Subudhi, H.N., Rao, G.J.N., 2012. Molecular analysis of native Manipur rice accessions for resistance against blast. African Journal of Biotechnology 11(6), 1321-1329. DOI: https://doi.org/10.5897/AJB11.2178. DOI: https://doi.org/10.5897/AJB11.2178

Panja, S., Biswas, R., Kar, R.K., Dey, P.C., Dey, N., 2023. Morpho-molecular characterization of ethnic Bora rice for conservation and breeding. Genetic Resources and Crop Evolution 70, 1853-1866. DOI: https://doi.org/10.1007/s10722-023-01541-8. DOI: https://doi.org/10.1007/s10722-023-01541-8

Qu, S., Liu, G., Zhou, B., Bellizzi, M., Zeng, L., Dai, L., Han, B., Wang, G.L., 2006. The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice. Genetics 172(3), 1901-1914. DOI: https://doi.org/10.1534/genetics.105.044891. DOI: https://doi.org/10.1534/genetics.105.044891

Ramkumar, G., Srinivasarao, K., Mohan, K.M., Sudarshan, I., Sivaranjani, A.K.P., Gopalakrishna, K., Neeraja, C.N., Balachandran, S.M., Sundaram, R.M., Prasad, M.S., Rani, N.S., Rama Prasad, A.M., Viraktamath, B.C., Madhav, M.S., 2011. Development and validation of functional marker targeting an InDel in the major rice blast disease resistance gene Pi54 (Pik-h). Molecular Breeding 27, 129-135. DOI: https://doi.org/10.1007/s11032-010-9538-6. DOI: https://doi.org/10.1007/s11032-010-9538-6

Sahu, C., Yadav, M.K., Panda, G., Aravindan, S., Umakanta, N., Raghu, S., Prabhukarthikeyan, S.R., Keerthana, U., Adak, T., Sharma, V., Mohanty, M.R., Rath, P.C., Jena, M., 2018. Morphological and molecular characterization of Magnaporthe oryzae causing rice blast disease in Odisha. ORYZA - An International Journal on Rice 55(3), 467-472. DOI: https://doi.org/10.5958/2249-5266.2018.00056.5. DOI: https://doi.org/10.5958/2249-5266.2018.00056.5

Shanmugam, A., Suresh, R., Ramanathan, A., Anandhi, P., Sassikumar, D., 2023. Unravelling genetic diversity of South Indian rice landraces based on yield and its components. Electronic Journal of Plant Breeding 14(1), 160-169. DOI: https://doi.org/10.37992/2023.1401.007. DOI: https://doi.org/10.37992/2023.1401.007

Sharma, T.R., Madhav, M.S., Singh, B.K., Shanker, P., Jana, T.K., Dalal, V., Pandit, A., Singh, A., Gaikwad, K., Upreti, H.C., Singh, N.K., 2005. High-resolution mapping, cloning and molecular characterization of the Pik-h gene of rice, which confers resistance to Magnaporthe grisea. Molecular Genetics and Genomics 274, 569-578. DOI: https://doi.org/10.1007/s00438-005-0035-2. DOI: https://doi.org/10.1007/s00438-005-0035-2

Sooklim, C., Chomnunti, P., Jantasuriyarat, C., Chukeatirote, E., Nilthong, R., Nilthong, S., 2022. Genetic diversity and population structure of blast resistance genes in Thai upland rice germplasm. European Journal of Plant Pathology 163(3), 587-599.

DOI: https://doi.org/10.1007/s10658-022-02499-6. DOI: https://doi.org/10.1007/s10658-022-02499-6

Thete, A.M., Dahat, D.V., Jambhale, V.M., Chaudhari, S.R., 2023. Molecular characterization of rice genotypes using molecular markers. International Journal of Plant & Soil Science 35(20), 284-301. DOI: https://doi.org/10.9734/ijpss/2023/v35i203809. DOI: https://doi.org/10.9734/ijpss/2023/v35i203809

Tian, D., Chen, Z., Chen, Z., Zhou, Y., Wang, Z., Wang, F., Chen, S., 2016. Allele-specific marker-based assessment revealed that the rice blast resistance genes Pi2 and Pi9 have not been widely deployed in Chinese indica rice cultivars. Rice 9, 19. DOI: https://doi.org/10.1186/s12284-016-0091-8. DOI: https://doi.org/10.1186/s12284-016-0091-8

Xu, X., Hayashi, N., Wang, C.T., Kato, H., Fujimura, T., Kawasaki, S., 2008. Efficient authentic fine mapping of the rice blast resistance gene Pik-h in the Pik cluster, using new Pik-h-differentiating isolates. Molecular Breeding 22, 289-299. DOI: https://doi.org/10.1007/s11032-008-9175-5. DOI: https://doi.org/10.1007/s11032-008-9175-5

Yadav, M.K., Aravindan, S., Ngangkham, U., Raghu, S., Prabhukarthikeyan, S.R., Keerthana, U., Marndi, B.C., Adak, T., Munda, S., Deshmukh, R., Pramesh, D., Samantaray, S., Rath, P.C., 2019. Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers. PLoS ONE 14(1), e0211061. DOI: https://doi.org/10.1371/journal.pone.0213566. DOI: https://doi.org/10.1371/journal.pone.0211061

Yi, G., Lee, S.K., Hong, Y.K., Cho, Y.C., Nam, M.H., Kim, S.C., Han, S.S., Wang, G.L., Hahn, T.R., Ronald, P.C., Jeon, J.S., 2004. Use of Pi5(t) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea. Theoretical and Applied Genetics 109, 978- 985. DOI: https://doi.org/10.1007/s00122-004-1707-8. DOI: https://doi.org/10.1007/s00122-004-1707-8

Downloads

Published

2023-12-13

How to Cite

[1]
Shanmugam, A. et al. 2023. Characterization of Traditional Rice Varieties for Leaf Blast Resistant Genes Pi5, Pi54, Pi9 and Pi2 using Gene Specific Markers. Research Biotica. 5, 4 (Dec. 2023), 158–161. DOI:https://doi.org/10.54083/ResBio/5.4.2023/158-161.

Issue

Section

Research Articles