TILLING: A Novel Reverse Genetics Technique for Screening of Gene Mutants in Plants

Abstract

TILLING is a newly developed general reverse genetics strategy which helps to locate an allelic series of induced point mutations in genes of interest and allows the rapid and inexpensive detection of induced point mutations in populations of physically or chemically mutagenized individuals. This approach works with a mismatch-specific endonuclease to detect induced or natural DNA polymorphisms in desired genes.